But of course, at the moment our IT is again hopelessly behind as everybody else is moving to WireGuard, which is conceptually easier and better performing. Hopefully, IT has learned the lesson that you cannot buy security with money. Then it appeared that Pulse Secure was just pretending to be secure. IT soon realized that the license fees would be too expensive to allow everybody to use a VPN and therefore our university was running two parallel VPN solutions: Pulse Secure and OpenVPN (the latter being free). After about 2 hours, I had downloaded and installed all updates and was even able to manually delete "Pulse Secure" ( Pulse Secure was the horrendously insecure "secure communication" tool that the university IT wanted the whole university to use a few years back.
#Macvector create reference file install#
After booting, I first needed to install an onslaught of updates, without which I was permanently bombarded with pop-ups that asked me to bin "Pulse Secure" and "navlibx". The 2013 Macbook had been upgraded to run macOS 10.15 (Catalina) perhaps a year ago when I last time used it.
I needed to use my old 2013 Macbook because GCK runs only on Mac and Windows, and all my PCs are running Linux. To open this file I needed to install GCK on one of my computers. Updating macOS and getting rid of constantly nagging pop-ups gci (Illustration) files cannot be opened by SnapGene. We had used GCK until 2013 when we replaced it with the much better designed and more capable SnapGene.
#Macvector create reference file how to#
But how to open it? The actual GCK sequence files can be opened by the contemporary DNA construct mapping and cloning software SnapGene. And since I never delete anything, the file was still in my "Documents/Sequences/GCK/pSecTag/pSecTagI" folder. gci file for the construction of pSecTagI, which does contain a detailed description of the cloning. However, it was possible to manually create so-called Illustration files (.gci file extension), which could be used to manually document complex cloning projects and to create figures for manuscripts or presentations.
GCK has had the peculiarity that it did not store the history of the construction.
Last Wednesday, I spent 4 hours of my working time opening one file created with GCK version 2.5 in 1999. We were at the "cutting edge" of technology because we used a software program called Gene Construction Kit (GCK) to keep track of our clonings. In 1999, I was working in Kari Alitalo's laboratory as a Ph.D. For a new cloning project, we needed to access the plasmid maps of an old construct of mine (pSecTagN2, which was a precursor of pMosaic, aka pSecTagI) which I had composed from many different sources in 1999.
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